background image
TNT technology has two major components:
First is a nanotechnology-based chip de-
signed to deliver cargo to adult cells in the
live body. Second is the design of specific
biological cargo for cell conversion.
This cargo, when delivered using the chip,
converts an adult cell from one type to an-
other, said first author Daniel Gallego-Perez,
an assistant professor of biomedical engi-
neering and general surgery who also was
a postdoctoral researcher in both Sen¡¯s and
Lee¡¯s laboratories.
TNT doesn¡¯t require any laboratory-based
procedures and may be implemented at the
point of care. The procedure is also non-
The cargo is delivered by zapping the device
with a small electrical charge that¡¯s barely
felt by the patient.
¡°The concept is very simple,¡± Lee said. ¡°As a
matter of fact, we were even surprised how
it worked so well.
In my lab, we have ongoing research trying to understand the mechanism
and do even better. So, this is the beginning, more to come.¡±
Researchers plan to start clinical trials next year to test this technology in
humans, Sen said.
Funding for this research was provided by Leslie and Abigail Wexner, Ohio
State¡¯s Center for Regenerative Medicine and Cell-Based Therapies and
Ohio State¡¯s Nanoscale Science and Engineering Center.
Journal Reference:
Daniel Gallego-Perez, Durba Pal, Subhadip Ghatak, Veysi Malkoc, Natalia
Higuita-Castro, Surya Gnyawali, Lingqian Chang, Wei-Ching Liao, Jun-
feng Shi, Mithun Sinha, Kanhaiya Singh, Erin Steen, Alec Sunyecz, Rich-
ard Stewart, Jordan Moore, Thomas Ziebro, Robert G. Northcutt, Michael
Homsy, Paul Bertani, Wu Lu, Sashwati Roy, Savita Khanna, Cameron Rink,
Vishnu Baba Sundaresan, Jose J. Otero, L. James Lee, Chandan K. Sen.
Topical tissue nano-transfection mediates non-viral stroma reprogramming
and rescue. Nature Nanotechnology, 2017; DOI: 10.1038/nnano.2017.134
Materials provided by Ohio State University Wexner Medical Center.)
Breakthrough Device Heals Organs With A Single Touch
Repairs tissue, restores function of aging tissue, organs, blood vessels, nerve cells
Mobile fishing app
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Chip does not stay with you;
Immune suppression not necessary
Written by Ohio State University Wexner Medical
Center, August 18, 2017: Researchers at The Ohio
State University Wexner Medical Center and Ohio
State¡¯s College of Engineering have developed
a new technology, Tissue Nanotransfection
(TNT), that can generate any cell type of
interest for treatment within the patient¡¯s own
This technology may be used to repair injured
tissue or restore function of aging tissue,
including organs, blood vessels and nerve cells.
Results of the regenerative medicine study are
published in the journal Nature Nanotechnology.
¡°By using our novel nanochip technology, injured
or compromised organs can be replaced.
We have shown that skin is a fertile land where
we can grow the elements of any organ that is
declining,¡± said Dr. Chandan Sen, director of Ohio
State¡¯s Center for Regenerative Medicine & Cell
Based Therapies.
2017 OCT/NOV #8-5
Dr. Chandan Sen co-led the study with L. James Lee, professor of chemi-
cal and biomolecular engineering with Ohio State¡¯s College of Engineer-
ing, in collaboration with Ohio State¡¯s Nanoscale Science and Engineering
Researchers studied mice and pigs in these experiments. In the study,
researchers were able to reprogram skin cells to become vascular cells in
badly injured legs that lacked blood flow. Within one week, active blood
vessels appeared in the injured leg, and by the second week, the leg was
In lab tests, this technology was also shown to reprogram skin cells in
the live body into nerve cells that were injected into brain-injured mice to
help them recover from stroke.
¡°This is difficult to imagine, but it is achievable, successfully working
about 98 percent of the time. With this technology, we can convert skin
cells into elements of any organ with just one touch.
This process only takes less than a second and is non-invasive, and then
you¡¯re off.
The chip does not stay with you, and the reprogramming of the cell
starts. Our technology keeps the cells in the body under immune surveil-
lance, so immune suppression is not necessary,¡± said Sen, who also is
executive director of Ohio State¡¯s Comprehensive Wound Center.